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Alco-Screen/Alco-Screen02 blood alcohol tester

Buy Alco-Screen/Alco-Screen02 blood alcohol tester

Principle1
It is well established that the concentration of alcohol in saliva is comparable to that of blood.2,3,4,5   The following figure shows the correlation between saliva and blood alcohol. The regression line equation was:

blood alcohol tester

The ALCO-SCREENTM/ALCO-SCREEN02 blood alcohol tester consists of a plastic strip with a reactive pad applied at the tip. The tip, on contact with solutions of alcohol, will rapidly turn shades of green to blue depending on the amount of alcohol present. The reactive pad employs a solid phase chemistry which uses the following highly specific enzyme reaction:

 

Specificity
The ALCO-SCREENTM/ALCO-SCREEN02 tester will react with methyl, ethyl, and allyl alcohols. ALCO-SCREENTM/ALCO-SCREEN02 tester will not react with substances having 5 or more carbons, nor with glycine, glycerol, or serine. This property is a result of the specificity of the alcohol oxidase enzyme extracted from yeast.1

Reagent Composition: (per tester unit)

Tetramethylbenzidine            		0.176 mg
Alcohol Oxidase (EC 1.1.3.1.3)  	         0.5 IU 
Peroxidase (EC 1.11.1.7)      		30 IU
Buffer                          		0.747 mg
Stabilizing Proteins            		0.19 ug

Interferences
The following substances may interfere with the ALCO-SCREENTM/ALCO-SCREEN02 tester stick when using samples other than saliva:

    Agents which enhance color development:
   
     Peroxides
        Strong oxidizers
    Agents which inhibit color development:
        Reducing Agents:
            Ascorbic acid
            Tannic Acid
   
         Pyrogallol
            Mercaptans and tosylates
            Oxalic acid
            Uric acid
   
     Bilirubin
   
     L-dopa
        L-methyldopa
   
     Methampyrone

The above-named substances do not normally appear in sufficient quantity in saliva to interfere with the tester. However, care must be taken that they are not introduced into the mouth during the 15 minute period preceding the test.

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