
Buy Alco-Screen/Alco-Screen02 blood alcohol tester
Principle1
It is well
established that the concentration of alcohol in saliva is comparable to
that of blood.2,3,4,5 The following figure shows the correlation
between saliva and blood alcohol. The regression line equation was:
The ALCO-SCREENTM/ALCO-SCREEN02 blood alcohol tester consists of a plastic strip with a reactive pad applied at the tip. The tip, on contact with solutions of alcohol, will rapidly turn shades of green to blue depending on the amount of alcohol present. The reactive pad employs a solid phase chemistry which uses the following highly specific enzyme reaction:
Specificity
The ALCO-SCREENTM/ALCO-SCREEN02
tester will react with methyl, ethyl, and allyl alcohols. ALCO-SCREENTM/ALCO-SCREEN02
tester will not react with substances having 5 or more carbons, nor with
glycine,
glycerol, or serine. This property is a result of the specificity of the alcohol
oxidase enzyme extracted from yeast.1
Reagent Composition: (per tester unit)
Tetramethylbenzidine 0.176 mg Alcohol Oxidase (EC 1.1.3.1.3) 0.5 IU Peroxidase (EC 1.11.1.7) 30 IU Buffer 0.747 mg Stabilizing Proteins 0.19 ug
Interferences
The following substances
may interfere with the ALCO-SCREENTM/ALCO-SCREEN02
tester stick when using samples other than saliva:
Agents which enhance color
development:
Peroxides
Strong oxidizers
Agents which inhibit color development:
Reducing Agents:
Ascorbic acid
Tannic Acid
Pyrogallol
Mercaptans
and tosylates
Oxalic acid
Uric acid
Bilirubin
L-dopa
L-methyldopa
Methampyrone
The above-named substances do not normally appear in sufficient quantity in saliva to interfere with the tester. However, care must be taken that they are not introduced into the mouth during the 15 minute period preceding the test.
Buy Alco-Screen/Alco-Screen02 blood alcohol tester
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